Prof. Nai-Chen Cheng’s group has developed the biomaterial-assisted and scaffold-free 3D culture technology for cell therapy.
Therapeutic mechanism of the ASC sheet FBS-cultured ASC sheets suppressed macrophage infiltration and exhibited an anti-scarring effect, thereby enhancing skin healing with superior quality of neo-dermis. (Yu et al. Acta Biomaterialia 2018) Employ HPL for the ASC sheet fabrication They investigated the effect of human platelet lysate (HPL) on extracellular matrix (ECM) deposition and cellular characteristics in ASC sheets, and successfully fabricated thicker ASC sheets with enhanced ECM deposition. (Cheng et al. Front. Cell Dev. Biol. 2020) Composite ASC spheroids and sheets for engineering scaffold-free biosystems Schematic diagram of the manufacturing process of a composite ASC spheroid sheet. (Yu et al. Stem Cell Research & Therapy 2022) Investigate the biological characteristics among ASC spheroids, sheets, and 2D ASCs In the spheroid sheet construct, ASC spheroids can augment the pro-angiogenesis capability of ASC sheets without the use of exogenous biomaterial or genetic manipulation. In the spheroid sheet construct, ASC spheroids can augment the pro-angiogenesis capability of ASC sheets without the use of exogenous biomaterial or genetic manipulation. (Yu et al. Stem Cell Research & Therapy 2022) GTP-compatible ASC sheets for clinical therapeutic purposes HPL-cultured ASC sheets combined with bio-cellulose membranes are being developed as a potential treatment for diabetic ulcers. (Yu et al. Stem Cell Research & Therapy 2022)